Methanol HPLC 2.5L

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Methanol HPLC (CH₃OH,) is one of the most commonly used organic modifiers in reversed-phase chromatography. Although the solvent offers certain advantages and disadvantages, one of Methanol’s key strengths, from a chromatographic perspective, is that it offers substantially different selectivity, and as such, are valuable for method development purposes. 

There are a range of organic solvents that are suitable for use as the organic modifier in reversed-phase liquid chromatography; in practice though, only a few are routinely used. When selecting an organic solvent, properties such as miscibility with water, polarity, UV cut-off, viscosity and safety are important to consider

Over time, methanol has become one of the organic modifiers of choice for numerous reversed-phase applications. As a solvent it is fully miscible with water and is compatible with common mobile phase additives as well as buffers (care should be taken when using buffer salts at high organic compositions). 

Although Methanol has advantages and disadvantages that should be taken into consideration, some of its key benefits are that it is affordable and less toxic than other organic modifiers. We offer Methanol HPLC in 2,5l bottles; purchase yours online today!

Methanol HPLC – Identification of Product
Chemical Code: HPLC-M1
Chemical Name: Methanol
Chemical Grade: HPLC
Chemical Formula: CH₄O
Chemical Weight: 32,04 g/mol
CAS No: 67-56-1
Chemical Synonyms: Methanol HPLC

Methanol HPLC – Hazards Identification
REACH No: 01-2119433307-44-XXXX
Signal Word: Danger
Supplemental Hazard Information:
Additional Hazard Information: No Data Available

Methanol HPLC – Hazards statements
H225 – Highly flammable liquid and vapour.H301 – Toxic if swallowed.
H311 – Toxic in contact with skin.
H331 – Toxic if inhaled.
H370 – Causes damage to organs.

Methanol HPLC – Precautionary statements
P210 – Keep away from heat/sparks/open flames/hot surfaces. No smoking.
P260 – Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P280 – Wear protective gloves/ protective clothing.
P301 + P310 – IF SWALLOWED: Immediately call a POISON CENTER or doctor/ physician.
P311 – Call a POISON CENTER or doctor/ physician.

Methanol HPLC – Composition of Chemical
Chemical Formula: CH₄O
EC No 1272/2008: 01-2119433307-44-XXXX

Methanol HPLC – First Aid Measures
General Advice: Consult a physician. Show this safety data sheet to the doctor in attendance.
If: Inhaled: If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
If: Skin Contact: Wash off with soap and plenty of water. Take victim immediately to hospital. Consult a physician.
If: Eye Contac: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
If: Swallowed: Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
Important Symptoms: Dizziness, Drowsiness, Metabolic acidosis, Blurred vision, Seizures, Coma, Blindness, Death.
Immediate Medical Attention: No Data Available

Methanol HPLC – Firefighting Measures
Extinguishing Media: Use Water spray, Alcohol-resistant foam, Dry chemical or Carbon Dioxide.
Hazards Arising: Carbon Oxides.
Advice for Firefighters: Wear self-contained breathing apparatus for firefighting if necessary.
Info for Firefighting: Use water spray to cool unopened containers.

Accidental Release Measures
Personal Precautions: Wear respiratory protection. Avoid breathing vapours, mist or gas.
Ensure adequate ventilation.
Remove all sources of ignition.
Evacuate personnel to safe areas.
Beware of vapours accumulating to form explosive concentrations.
Vapours can accumulate in low areas.
Enviromental Precautions: Prevent further leakage or spillage if safe to do so.
Do not let product enter drains.
Method for Containment: Contain spillage, and then collect with an electrically protected vacuum cleaner or by wet-brushing and place in container for disposal according to local regulations.

Handling and Storage
Personal Precautions: Avoid contact with skin and eyes.
Avoid inhalation of vapour or mist.
Keep away from sources of ignition – No smoking.
Take measures to prevent the build up of electrostatic charge.
Enviromental Precautions: Keep container tightly closed in a dry and well-ventilated place.
Containers which are opened must be carefully resealed and kept upright to prevent leakage.
Keep in a cool place.
Exposure Controls | Personal Protection
Derived No Effect Level (DNEL)
Workers | Application Area | Exposure Routes | Health Effect | Value
Workers – Skin contact – Long-term systemic effects – 40mg/kg BW/d.
Workers – Skin contact – Acute systemic effects – 40mg/kg BW/d.
Workers – Inhalation – Acute systemic effects – 260 mg/m3.
Workers – Inhalation – Acute local effects – 260 mg/m3.
Workers – Inhalation – Long-term systemic effects – 260 mg/m3.
Workers – Inhalation – Long-term local effects – 260 mg/m3.
Consumers | Application Area | Exposure Routes | Health Effect | Value
Consumers – Skin contact – Long-term systemic effects – 8mg/kg BW/d.
Consumers – Ingestion – Long-term systemic effects – 8mg/kg BW/d.
Consumers – Skin contact – Acute systemic effects – 8mg/kg BW/d.
Consumers – Ingestion – Acute systemic effects – 8mg/kg BW/d.
Consumers – Inhalation – Acute systemic effects 50 mg/m3.
Consumers – Inhalation – Acute local effects 50 mg/m3.
Consumers – Inhalation – Long-term systemic effects – 50 mg/m3.
Consumers – Inhalation – Long-term local effects – 50 mg/m3.
Predicted No Effect Concentration (PNEC)
Soil – 23,5 mg/kgMarine water – 15,4 mg/l
Fresh water – 154 mg/l
Fresh water sediment – 570,4 mg/kg
Onsite sewage treatment plant – 100 mg/kg
Engineering Controls: Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
Eye/Face Protection: Face shield and safety glasses. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).
Skin Protection: Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching gloves outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it.
Full contact –
Material: Butyl-rubber. Minimum layer thickness: 0,3 mm.
Break through time: 480 min. Material tested: Butoject®.
Splash contact –
Material: Nitrile rubber. Minimum layer thickness: 0,4 mm.
Break through time: 31 min. Material tested: Camatril®.
Data source: KCL GmbH, D-36124. Test method: EN374.
If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact the supplier of the CE approved gloves. This recommendation is advisory only and must be evaluated by an industrial hygienist and safety officer familiar with the specific situation of anticipated use by our customers. It should not be construed as offering an approval for any specific use scenario.
Body Protection: Complete suit protecting against chemicals. Flame retardant anti-static protective clothing. The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.
Respiratory Protection: Where risk assessment shows air-purifying respirators are appropriate use a full-face respirator with multipurpose combination (US) or type AXBEK (EN 14387) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).

Physical and Chemical Properties
Appearance: Clear, Colourless, mobile liquid
Odour: Pungent
Odour Threshold: No Data Available
pH: No Data Available
Melting Point: Melting Point/Range: -98 °C
Boiling Point: 64,7 °CFlash Point: 9,7 °C (Closed Cup).
Evaporation: No Data Available
Flammability: No Data Available
Upper/Lower Flammability or Explosive Limits: Upper explosion limit: 36 %(V,
Lower explosion limit: 6 %(V).
Vapour pressure: 130,3 hPa at 20,0 °C,
546,6 hPa at 50,0 °C,
169,27 hPa at 25,0 °C.
Vapour density: 1,11
Relative density: 0,791 g/mL at 25 °C
Water solubility: Completely Miscible
Partition Coefficien: Log Pow: -0,77
Auto-ignition Temperature: 455,0 °C at 1.013 hPa
Decomposition Temperature: No Data Available
Viscosity: No Data Available
Explosive properties: No Data Available
Oxidizing properties: The substance or mixture is not classified as oxidizing.
Other Safety Info: Minimum ignition energy: 0,14 mJ.
Conductivity: < 1 ?S/cm,
Relative vapour density: 1,11.
Stability and Reactivity
Reactivity: No Data Available
Chemical Stability: Stable under recommended storage conditions.
Possibility of hazardous reactions: No Data Available
Conditions to Avoid: Heat, flames and sparks, Extremes of temperature and direct sunlight.
Incompatible Materials: Acid Chlorides, Acid Anhydrides, Oxidizing agents, Alkali metals, Reducing agents, Acids.
Hazardous Decomposition Products: No Data Available

Toxicological Information
Acute Toxicity: LDLO Oral – Human – 143 mg/kg
Remarks: Lungs, Thorax, or Respiration: Dyspnea. Ingestion may cause gastrointestinal irritation, nausea, vomiting and diarrhoea.
LD50 Oral – Rat – 1.187 – 2.769 mg/kg
LC50 Inhalation – Rat – 4 h – 128,2 mg/l
LC50 Inhalation – Rat – 6 h – 87,6 mg/lLD50 Dermal – Rabbit – 17.100 mg/kg
Skin Corrosion/Irritation: Rabbit
Result: No skin irritation
Serious Eye damage | Eye Irritation: Rabbit
Result: No eye irritation
Cell Mutagenicity: Ames test – S. typhimurium – Result: negative.
In vitro assay – Fibroblast – Result: negative – Mutation in mammalian somatic cells.
Mutagenicity (in vivo mammalian bone-marrow cytogenetic test, chromosomal analysis) – Mouse – male and female – Result: negative
Carcinogenicity: IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.
Reproductive toxicity: Damage to fetus not classifiable
Fertility classification not possible from current data.
Specific Target Organ Toxicity – Single Exposure: Causes damage to organs.
Specific Target Organ Toxicity – Repeated Exposure: The substance or mixture is not classified as specific target organ toxicant, repeated exposure.
Aspiration Hazard: No aspiration toxicity classification.

Ecological Information
Ecological Toxicity: Toxicity to fish:
Mortality LC50 – Lepomis macrochirus (Bluegill) – 15.400,0 mg/l – 96 h
NOEC – Oryzias latipes – 7.900 mg/l – 200 h.
Toxicity to daphnia and other aquatic invertebrates:
EC50 – Daphnia magna (Water flea) – > 10.000,00 mg/l – 48 h.
Toxicity to algae:
Growth inhibition EC50 – Scenedesmus capricornutum (fresh water algae) – 22.000,0 mg/l – 96 h.
Ecological Persistence and degradability: Biodegradability:
Aerobic – Exposure time 5 d – Result: 72 % – rapidly biodegradable.
Biochemical Oxygen Demand – (BOD) – 600 – 1.120 mg/g.
Chemical Oxygen Demand – (COD) – 1.420 mg/g.
Theoretical Oxygen Demand – 1.500 mg/g
Bioaccumulative Potential: Bio-accumulation: Cyprinus carpio (Carp) – 72 d at 20 °C – 5 mg/l.
Bio-concentration factor (BCF): 1,0Mobility in Soil: Will not adsorb on soil.
Results of PBT and vPvB Assessment: PBT/vPvB assessment not available as chemical safety assessment not required/not conducted.
Other Adverse Effect: Avoid release to the environment.
Stability in water – at 19 °C83 – 91 % – 72 h. Remarks: Hydrolyses on contact with water. Hydrolyses readily.

Disposal Considerations
Waste Treatment Methods: Product
Burn in a chemical incinerator equipped with an afterburner and scrubber but exert extra care in igniting as this material is highly flammable. Offer surplus and non-recyclable solutions to a licensed disposal company.
Contaminated packaging – Dispose of as unused product.

Transport Information
UN Number: ADR/RID: 1230
IMDG: 1230
IATA: 1230
UN Shipping Hazard: ADR/RID: METHANOL
IATA: Methanol
Transport Hazard Class: ADR/RID: 3 (6.1)
IMDG: 3 (6.1)
IATA: 3 (6.1)
Packaging Group: ADR/RID: II
Enviromental Hazards: ADR/RID: no
IMDG Marine pollutant: no
IATA: no
Special Precautions: No Data Available

Regulatory Information
Safety, Health and environmental regulations: This safety datasheet complies with the requirements of Regulation (EC) No. 1907/2006.
International Chemical Weapons Convention (CWC) Schedules of Toxic Chemicals and Precursors: Neither banned nor restricted.
REACH – Restrictions on the manufacture, placing on the market and use of certain dangerous substances, preparations and articles (Annex XVII):
Neither banned nor restricted.
Regulation (EC) No 649/2012 of the European Parliament and the Council concerning the export and import of dangerous chemicals:
Neither banned nor restricted.
REACH – Candidate List of Substances of Very High Concern for Authorization (Article 59): This product does not contain substances of very high concern (Regulation (EC) No. 1907/2006 (REACH), Article 57). REACH – List of substances subject to authorization (Annex XIV): Neither banned nor restricted.
Regulation (EC) No 1005/2009 on substances that deplete the ozone layer: Neither banned nor restricted.
Regulation (EC) No 850/2004 on persistent organic pollutants: Neither banned nor restricted.
Chemical Safety Assessment: A Chemical Safety Assessment has been carried out for this substance.
Additional Info: RTECS: PC1400000.
Effects due to ingestion may include: Headache, Dizziness, Drowsiness, Metabolic acidosis, Coma, Seizures.
Methyl alcohol may be fatal or cause blindness if swallowed.

The information stated above is considered to be correct, but does not claim to be inclusive and shall only be used as a guideline. The information provided by this document is confirmed by our continuous updating of knowledge and adheres to the products appropriate safety precautions. It does not represent any guarantee of the product’s
properties. RLS Chemicals and its Associates shall not be held accountable for any damage’s consequent of handling the above product

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